Zbornik Matice Srpske za Prirodne Nauke (Jan 2019)

Identification and phylogenetic analysis of Fusarium proliferatum isolated from elephant garlic Allium ampeloprasum L.

  • Ignjatov Maja V.,
  • Vlajić Slobodan A.,
  • Milošević Dragana N.,
  • Nikolić Zorica T.,
  • Tamindžić Gordana D.,
  • Gvozdanović-Varga Jelica M.,
  • Ivanović Žarko S.

DOI
https://doi.org/10.2298/ZMSPN1937049I
Journal volume & issue
Vol. 2019, no. 137
pp. 49 – 55

Abstract

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Symptoms of cloves rot of A. ampeloprasum were noticed during 2018 in storage conditions. 16 isolates were obtained (AMP1-AMP16) and according to morphological and cultural characteristics they belong to F. proliferatum (sex. stage Gibberella intermedia), species of Gibberella fujikuroi complex. To confirm morphological identifica­tion, total genomic DNA was extracted from mycelium of the 16 isolates by amplification of TEF-1α gene, using polymerase chain reaction (PCR) that was performed with the primer pair EF1 and EF2. Results presented in this article clearly indicated that the new host of Fusarium proliferatum as the causal agent of cloves rot is „elephant garlic“ Allium ampeloprasum. Pathogenicity test was confirmed on Allium ampeloprasum cloves. Pathogenicity assays revealed that all isolates caused symptoms on tested Allium spp., like naturally infected cloves. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. TR31030]

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