Targeting nanoparticles to lung cancer-derived A549 cells based on changes on interstitial stiffness in biomimetic models

Afia Ibnat Kohon; Kun Man; Ala Hessami; Katelyn Mathis; Jade Webb; Joanna Fang; Parsa Radfar; Yong Yang; Brian Meckes

iScience (Oct 2024)

Targeting nanoparticles to lung cancer-derived A549 cells based on changes on interstitial stiffness in biomimetic models

Afia Ibnat Kohon,
Kun Man,
Ala Hessami,
Katelyn Mathis,
Jade Webb,
Joanna Fang,
Parsa Radfar,
Yong Yang,
Brian Meckes

Affiliations

Afia Ibnat Kohon: Department of Biomedical Engineering, University of North Texas, 3940 N Elm St., Denton, TX 76207, USA; BioDiscovery Institute, University of North Texas, 1155 Union Circle, Denton, TX 76203-5017, USA
Kun Man: Department of Biomedical Engineering, University of North Texas, 3940 N Elm St., Denton, TX 76207, USA
Ala Hessami: Department of Biomedical Engineering, University of North Texas, 3940 N Elm St., Denton, TX 76207, USA
Katelyn Mathis: Department of Biomedical Engineering, University of North Texas, 3940 N Elm St., Denton, TX 76207, USA; BioDiscovery Institute, University of North Texas, 1155 Union Circle, Denton, TX 76203-5017, USA
Jade Webb: Department of Biomedical Engineering, University of North Texas, 3940 N Elm St., Denton, TX 76207, USA
Joanna Fang: Department of Biomedical Engineering, University of North Texas, 3940 N Elm St., Denton, TX 76207, USA
Parsa Radfar: Department of Biomedical Engineering, University of North Texas, 3940 N Elm St., Denton, TX 76207, USA
Yong Yang: Department of Biomedical Engineering, University of North Texas, 3940 N Elm St., Denton, TX 76207, USA; Corresponding author
Brian Meckes: Department of Biomedical Engineering, University of North Texas, 3940 N Elm St., Denton, TX 76207, USA; BioDiscovery Institute, University of North Texas, 1155 Union Circle, Denton, TX 76203-5017, USA; Corresponding author

Journal volume & issue: Vol. 27, no. 10
p. 111015

Abstract

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Summary: The mechanical properties and forces of the extracellular environment modulate alveolar epithelial cell behavior. To model cancer/fibrosis associated stiffening and dynamic stretch, a biomimetic device was developed that imitates the active forces in the alveolus, while allowing control over the interstitial matrix stiffness. Alveolar epithelial A549 cancer cells were cultured on the devices and their transcriptome was profiled with RNA sequencing. Pathway analysis showed soft materials upregulated the expression of proteoglycans associated with cancer. Consequently, liposomes were modified with peptides targeting heparan sulfate and chondroitin sulfates of the cell surface glycocalyx. Chondroitin sulfate A targeting improved uptake in cells seeded on stiff biomimetic devices, which is attributed to increased chondroitin sulfate proteoglycan localization on cell surfaces in comparison to cells grown on soft devices. These results demonstrate the critical role that mechanical stiffness and stretch play in the alveolus and the importance of including these properties in nanotherapeutic design.

Published in iScience

ISSN: 2589-0042 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science
Website: http://www.cell.com/iscience/home

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