Validation data of a rabbit antiserum and affinity purified polyclonal antibody against the N-terminus of human GPR30

Mohan C. Manjegowda; Paridhi Singhal Gupta; Anil M. Limaye

Data in Brief (Jun 2016)

Validation data of a rabbit antiserum and affinity purified polyclonal antibody against the N-terminus of human GPR30

Mohan C. Manjegowda,
Paridhi Singhal Gupta,
Anil M. Limaye

Affiliations

Mohan C. Manjegowda: Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India
Paridhi Singhal Gupta: Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India
Anil M. Limaye: Corresponding author.; Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India

Journal volume & issue: Vol. 7
pp. 1015 – 1020

Abstract

Read online

Rabbit antiserum was generated against the N-terminus of human GPR30 followed by peptide affinity purification. In this article, the methodology used and validation data are presented. The peptide affinity purified polyclonal antibody specifically detects human GPR30 in ELISA and on western blots of total protein prepared from human breast cancer cell lines. Keywords: GPR30, Antiserum, Affinity purified antibody, Western blotting

Published in Data in Brief

ISSN: 2352-3409 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Medicine: Medicine (General): Computer applications to medicine. Medical informatics; Science: Science (General)
Website: http://www.journals.elsevier.com/data-in-brief/

About the journal