Biorestauration and phytoremediation of soil impacted by waste residual oil

Abstract

In soil spill by 75000 ppm of waste motor oil (WMO) a mix of hydrocarbons (HICO) aliphatic, aromatic is relatively high according to Mexican regulation NOM-138-SEMARNAT/SSA1-2012 (NOM-138) which indicate that in soil the maximum limit accepted is 4400 ppm of HICO. WMO is an environmental problem because it´s causes lost soil´s fertility. The objectives of this research were a) biostimulation (BIS) of soil by 75000 ppm of WMO by mineral solution (MISO) and Vicia sativa as green manure (GEMA), b) phytoremediation (PHYTO) with Sorghum bicolor potencies by B. cepacia and P. chrysogenum for reducing WMO at concentration level value below the maximum accepted by the NOM-138. The results showed that BIS with MISO and V. sativa as GEMA reduced WMO from 75000 to 36700 ppm. Then PYTHO by S. bicolor potencies with B. vietnamiensis and P. chrysogenum decreased WMO from 36700 ppm to 790 ppm concentration value below to maximum accepted by NOM-138. Those results indicated that enrichment by MISO and GEMA induced WHO partial mineralization by heterotrophic aerobic native microorganisms, then PYTHO by S. bicolor and B. cepacia/P. chrysogenum completed its biorestauration by decreasing WMO level at below the highest accepted the Mexican environmental regulation NOM-138.

Keywords

• soil
• wmo
• mineral solution
• v. sativa
• s. vulgare
• b. vietnamiensis
• p. chrysogenum
• nom-138