Separations (Nov 2021)

Isolation and Identification of Non-Conjugated Linoleic Acid from Processed <i>Panax ginseng</i> Using LC-MS/MS and <sup>1</sup>H-NMR

  • Tae-Kyung Kim,
  • Changsuk Lee,
  • Taek-Hee Nam,
  • Yong-Ki Seo,
  • Kyeong-Soo Kim,
  • Kimoon Kang,
  • Seok-Hun Yun,
  • Seok-Hwan Yoon,
  • Jaeho Jeong

DOI
https://doi.org/10.3390/separations8110208
Journal volume & issue
Vol. 8, no. 11
p. 208

Abstract

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Black ginseng exhibits numerous pharmacological activities due to higher and more diverse ginsenosides than unprocessed white ginseng. The ginsenoside derivatives have been investigated in order to determine their chemical structures and pharmacological activities. We found a peak which was increased 10-fold but unidentified in the methanol extracts of a black ginseng product. The unknown peak was tracked and identified as linoleic acid rather than a ginsenoside derivative using liquid chromatography–tandem mass spectrometry (LC-MS/MS) and nuclear magnetic resonance (NMR) spectroscopy. NMR analysis confirmed no presence of conjugated linoleic acids. Ginsenoside profiles and linoleic acid contents in black ginseng products were quantified using LC-MS/MS. Linoleic acid content was more directly proportional to the number of applied thermal cycles in the manufacturing process than any ginsenosides.

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