Intracranial delivery of antisense locked nucleic acids for silencing microRNAs in the mouse brain

Abstract

Summary: Antisense locked nucleic acid (LNA) technology has been widely used for silencing microRNAs with enhanced specificity and efficiency. In this protocol, we first describe the procedure for targeted intracranial delivery of LNAs to silence microRNAs specifically in the mouse brain. We then detail the steps to isolate RNA and protein from mouse brain, followed by using RT-PCR and Western blotting to confirm microRNA silencing. This noninvasive approach can only be applied to mouse brain to specifically target silencing of microRNAs.For complete details on the use and execution of this protocol, please refer to Sharma et al. (2021).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Keywords

• Model organisms
• Molecular biology
• Gene expression
• Neuroscience