SUSCEPTIBILIDADE DE Chrysomya albiceps A Beauveria bassiana EM CONDIÇÕES DE LABORATÓRIO SUSCEPTIBILITY OF CHRYSOMYA ALBICEPS TO BEAUVERIA BASSIANA IN LABORATORY CONDITION

Abstract

<span><p align="justify">Chrysomya albiceps (Wiedemann) é um importante vetor de doenças de animais e humanos, e também causador de miíases secundárias. Avaliou-se a patogenicidade de <em>Beauveria bassiana</em><strong> (</strong>Balsamo) Vuillemin (URM 3447), <em>in vitro</em>, sobre ovos, larvas e adultos em condições de laboratório climatizado sob a temperatura de 28 ± 1 °C. Foram feitas suspensões de conídios (10⁴, 10⁵, 10⁶, 10⁷ e 10⁸ conídios.mL^-1), para análise dos parâmetros biológicos. No bioensaio com ovos, o percentual de eclosão variou de 84,81% a 97,22%. No bioensaio com larvas, o período de pré-pupa variou de 1 a 1,04 e de pupa, de 5,00 a 5,09 dias; o percentual de emergência de adultos a partir de larvas tratadas variou de 30,88% a 80,82%. No bioensaio com adultos, a longevidade para machos e fêmeas foi de 4,51 a 6,77 e 10,19 a 14,67 dias, respectivamente; a mortalidade acumulada no 7° dia variou de 47,50 % a 81,20% para machos e de 23,70 % a 55,00% dias para fêmeas; o período de postura variou de 10 a 17,75 dias e o percentual de larvas eclodidas a partir de fêmeas infectadas variou de 71,39% a 91,25%.</p><p align="justify"> </p><p align="justify">PALAVRAS-CHAVES:<strong> </strong>Fungo entomopatogênico, parâmetros biológicos, patogenicidade.</p></span> <span><p align="justify">Chrysomya albiceps (Wiedemann) is an important vector of animal and diseases as well as causer of secondary myiasis. In order to minimize the effect of the problems caused by <em>C. albiceps</em>, the pathogenicity of the <em>Beauveria bassiana</em><strong> (</strong>Balsamo) Vuillemin (URM 3447), in vitro, over eggs, larvae and adults in acclimatized laboratorial conditions under the temperature of 28 ± 1 °C was evaluated. Suspensions of conidia (10⁴,10⁵,10⁶, 10⁷ and 10⁸ conidia.mL^-1) were done, for the analysis of the biological parameters. In the bioassay with eggs, the percentage of eclosion varied from 84.81 to 97.22%. In the bioassay with larvae, pre-pupa period varied from 1 to 1.04 and of pupa, from 5 to 5.09 days; the percentage of adult emergency from treated larvae varied from 30.88 to 80.82%. As to the bioassay with adults, the longevity for male and female was of 4.51 to 6.77 and 10.19 to 14.67 days, respectively; the accumulated mortality death on the 7^th day varied from 47.50 to 81.20% for males and of 23.70 to 55.00% for females; the period of posture varied from 10 to17.75 days and the percentage of larvae appeared from females infected varied from 71.39 to 91.25%.</p><p align="justify"> </p><p align="justify">KEY WORDS: Biological parameters, entomopathogenic fungus, pathogenicity.</p></span>