Generation of induced pluripotent stem cell line from a patient suffering from arterial calcification due to deficiency of CD73 (ACDC)

Dipti Tripathi; Amit Manhas; Chikage Noishiki; David Wu; Shaunak Adkar; Karim Sallam; Eri Fukaya; Nicholas J. Leeper; Nazish Sayed

Stem Cell Research (Mar 2024)

Generation of induced pluripotent stem cell line from a patient suffering from arterial calcification due to deficiency of CD73 (ACDC)

Dipti Tripathi,
Amit Manhas,
Chikage Noishiki,
David Wu,
Shaunak Adkar,
Karim Sallam,
Eri Fukaya,
Nicholas J. Leeper,
Nazish Sayed

Affiliations

Dipti Tripathi: Baszucki Family Vascular Surgery Biobank, Stanford University School of Medicine, CA, USA; Division of Vascular Surgery, Department of Surgery, Stanford University School of Medicine, CA, USA
Amit Manhas: Stanford Cardiovascular Institute, Stanford University School of Medicine, CA, USA; Division of Cardiovascular Medicine, Stanford University School of Medicine, CA, USA
Chikage Noishiki: Division of Vascular Surgery, Department of Surgery, Stanford University School of Medicine, CA, USA; Stanford Cardiovascular Institute, Stanford University School of Medicine, CA, USA
David Wu: Division of Vascular Surgery, Department of Surgery, Stanford University School of Medicine, CA, USA; Stanford Cardiovascular Institute, Stanford University School of Medicine, CA, USA
Shaunak Adkar: Baszucki Family Vascular Surgery Biobank, Stanford University School of Medicine, CA, USA; Division of Vascular Surgery, Department of Surgery, Stanford University School of Medicine, CA, USA; Stanford Cardiovascular Institute, Stanford University School of Medicine, CA, USA
Karim Sallam: Stanford Cardiovascular Institute, Stanford University School of Medicine, CA, USA; Division of Cardiovascular Medicine, Stanford University School of Medicine, CA, USA
Eri Fukaya: Division of Vascular Surgery, Department of Surgery, Stanford University School of Medicine, CA, USA
Nicholas J. Leeper: Division of Vascular Surgery, Department of Surgery, Stanford University School of Medicine, CA, USA; Stanford Cardiovascular Institute, Stanford University School of Medicine, CA, USA; Division of Cardiovascular Medicine, Stanford University School of Medicine, CA, USA
Nazish Sayed: Baszucki Family Vascular Surgery Biobank, Stanford University School of Medicine, CA, USA; Division of Vascular Surgery, Department of Surgery, Stanford University School of Medicine, CA, USA; Stanford Cardiovascular Institute, Stanford University School of Medicine, CA, USA; Corresponding author at: 240 Pasteur Drive, 3551 Biomedical Innovations Building, Stanford, CA 95304, USA.

Journal volume & issue: Vol. 75
p. 103285

Abstract

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Arterial calcification due to deficiency of CD73 (ACDC) is an adult onset, rare genetic vascular disorder signified by calcium deposition in lower extremity arteries and joints of hands and feet. Mutations in NT5E gene has been shown to be responsible for the inactivation of enzyme CD73 causing calcium buildup. Here, we report a iPSC line generated from a patient showing signs of ACDC and carrying a missense mutation in NT5E (c.1126A→G,p.T376A) gene. This iPSC line shows normal morphology, pluripotency, karyotype, and capability to differentiate into three germ layers, making it useful for disease modeling and investigating pathological mechanisms of ACDC.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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