Communications Biology (Aug 2021)

Identification, mapping and relative quantitation of SARS-CoV-2 Spike glycopeptides by Mass-Retention Time Fingerprinting

  • Rod Chalk,
  • William E. P. Greenland,
  • Tiago Moreira,
  • Jesse Coker,
  • Shubhashish M. M. Mukhopadhyay,
  • Eleanor Williams,
  • Charlotte Manning,
  • Tina Bohstedt,
  • Rama McCrorie,
  • Alejandra Fernandez-Cid,
  • Nicola A. Burgess-Brown

DOI
https://doi.org/10.1038/s42003-021-02455-w
Journal volume & issue
Vol. 4, no. 1
pp. 1 – 8

Abstract

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Mapping multiple glycan species to the same peptide and location from shared retention time of glycopeptides with MS and HPLC, Chalk, Greenland et al. construct a detailed mass-retention time database for Spike protein glycopeptides. This allows any mass LC-MS laboratory to reliably identify and quantify Spike protein glycopeptides from a single overnight elastase protein digest in less than 90 minutes.