Journal of Bone and Joint Infection (Feb 2024)

Potential value of a rapid syndromic multiplex PCR for the diagnosis of native and prosthetic joint infections: a real-world evidence study

  • S. Pascual,
  • B. Noble,
  • N. Ahmad-Saeed,
  • C. Aldridge,
  • S. Ambretti,
  • S. Amit,
  • R. Annett,
  • S. A. O'Shea,
  • A. M. Barbui,
  • G. Barlow,
  • L. Barrett,
  • M. Berth,
  • A. Bondi,
  • N. Boran,
  • S. E. Boyd,
  • C. Chaves,
  • M. Clauss,
  • P. Davies,
  • I. T. Dianzo-Delgado,
  • J. Esteban,
  • S. Fuchs,
  • L. Friis-Hansen,
  • D. Goldenberger,
  • A. Kraševac Glaser,
  • J. O. Groonroos,
  • I. Hoffmann,
  • T. Hoffmann,
  • H. Hughes,
  • M. Ivanova,
  • P. Jezek,
  • G. Jones,
  • Z. Ceren Karahan,
  • C. Lass-Flörl,
  • F. Laurent,
  • L. Leach,
  • M. L. Horsbøll Pedersen,
  • C. Loiez,
  • M. Lynch,
  • R. J. Maloney,
  • M. Marsh,
  • O. Milburn,
  • S. Mitchell,
  • L. S. P. Moore,
  • L. Moffat,
  • M. Murdjeva,
  • M. E. Murphy,
  • D. Nayar,
  • G. Nigrisoli,
  • F. O'Sullivan,
  • B. Öz,
  • T. Peach,
  • C. Petridou,
  • M. Prinz,
  • M. Rak,
  • N. Reidy,
  • G. M. Rossolini,
  • A.-L. Roux,
  • P. Ruiz-Garbajosa,
  • K. Saeed,
  • L. Salar-Vidal,
  • C. Salas Venero,
  • M. Selvaratnam,
  • E. Senneville,
  • P. Starzengruber,
  • B. Talbot,
  • V. Taylor,
  • R. Trebše,
  • D. Wearmouth,
  • B. Willinger,
  • M. Wouthuyzen-Bakker,
  • B. Couturier,
  • F. Allantaz

DOI
https://doi.org/10.5194/jbji-9-87-2024
Journal volume & issue
Vol. 9
pp. 87 – 97

Abstract

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Introduction: The BIOFIRE Joint Infection (JI) Panel is a diagnostic tool that uses multiplex-PCR testing to detect microorganisms in synovial fluid specimens from patients suspected of having septic arthritis (SA) on native joints or prosthetic joint infections (PJIs). Methods: A study was conducted across 34 clinical sites in 19 European and Middle Eastern countries from March 2021 to June 2022 to assess the effectiveness of the BIOFIRE JI Panel. Results: A total of 1527 samples were collected from patients suspected of SA or PJI, with an overall agreement of 88.4 % and 85 % respectively between the JI Panel and synovial fluid cultures (SFCs). The JI Panel detected more positive samples and microorganisms than SFC, with a notable difference on Staphylococcus aureus, Streptococcus species, Enterococcus faecalis, Kingella kingae, Neisseria gonorrhoeae, and anaerobic bacteria. The study found that the BIOFIRE JI Panel has a high utility in the real-world clinical setting for suspected SA and PJI, providing diagnostic results in approximately 1 h. The user experience was positive, implying a potential benefit of rapidity of results' turnover in optimising patient management strategies. Conclusion: The study suggests that the BIOFIRE JI Panel could potentially optimise patient management and antimicrobial therapy, thus highlighting its importance in the clinical setting.