STAR Protocols (Jun 2021)

Protocol for automated production of human stem cell derived liver spheres

  • Jose Meseguer-Ripolles,
  • Alvile Kasarinaite,
  • Baltasar Lucendo-Villarin,
  • David C. Hay

Journal volume & issue
Vol. 2, no. 2
p. 100502

Abstract

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Summary: This protocol describes how to produce human liver spheres from pluripotent stem cell-derived hepatic progenitors, endothelial cells, and hepatic stellate cells. Liver spheres form by self-assembly in microwells, generating up to 73 spheres per well of a 96-well plate. This process was automated using liquid handling and pipetting systems, permitting cost-effective scale-up and reducing sphere variability. In its current form, this system provides a powerful tool to generate human liver tissue for disease modeling and drug screening.For complete details on the use and execution of this protocol, please refer to Lucendo-Villarin et al. (2020) (https://doi.org/10.1088/1758-5090/abbdb2).

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