Detection of Periodontal Pathogens Based on an Integrated Continuous Flow PCR and Capillary Electrophoresis Microfluidic Chip

Bo Yang; Jiaxin Huang; Chunxian Tao; Zhenqing Li; Dawei Zhang; Yoshinori Yamaguchi

doi:10.3390/separations10040271

Separations (Apr 2023)

Detection of Periodontal Pathogens Based on an Integrated Continuous Flow PCR and Capillary Electrophoresis Microfluidic Chip

Bo Yang,
Jiaxin Huang,
Chunxian Tao,
Zhenqing Li,
Dawei Zhang,
Yoshinori Yamaguchi

Affiliations

Bo Yang: Engineering Research Center of Optical Instrument and System, Shanghai Environmental Biosafety Instruments and Equipment Engineering Technology Research Center, School of Optoelectronic Information and Computer Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China
Jiaxin Huang: Engineering Research Center of Optical Instrument and System, Shanghai Environmental Biosafety Instruments and Equipment Engineering Technology Research Center, School of Optoelectronic Information and Computer Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China
Chunxian Tao: Engineering Research Center of Optical Instrument and System, Shanghai Environmental Biosafety Instruments and Equipment Engineering Technology Research Center, School of Optoelectronic Information and Computer Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China
Zhenqing Li: Engineering Research Center of Optical Instrument and System, Shanghai Environmental Biosafety Instruments and Equipment Engineering Technology Research Center, School of Optoelectronic Information and Computer Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China
Dawei Zhang: Engineering Research Center of Optical Instrument and System, Shanghai Environmental Biosafety Instruments and Equipment Engineering Technology Research Center, School of Optoelectronic Information and Computer Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China
Yoshinori Yamaguchi: Graduate School of Engineering, Osaka University, Osaka 565-0871, Japan

DOI: https://doi.org/10.3390/separations10040271
Journal volume & issue: Vol. 10, no. 4
p. 271

Abstract

Read online

Continuous-flow PCR (CF-PCR) can realize rapid DNA amplification because of the high temperature variation rate. However, off-line detection methods for PCR may induce cross contamination. To overcome this problem, we herein fabricated an integrated CF-PCR and electrophoresis microfluidic chip. The optimal voltage applied in the electrophoresis part of the microfluidic chip was achieved by simulation in COMSOL. Coating the inside wall of the microchannel can inhibit electroosmotic flow and improve the resolution for DNA fragments. The temperature distribution of the serpentine part can meet the PCR and has no obvious suppressive effect on sample separation. Finally, we have performed the amplification of target genes for Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola and detected the corresponding PCR products in the microfluidic chip within 11 min. Such work provides a new method for the rapid detection of bacteria.

Published in Separations

ISSN: 2297-8739 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Physics; Science: Chemistry
Website: http://www.mdpi.com/journal/separations

About the journal

Abstract

Keywords