De novo strategy with engineering anti-Kasha/Kasha fluorophores enables reliable ratiometric quantification of biomolecules

Limin Shi; Chenxu Yan; Zhiqian Guo; Weijie Chi; Jingle Wei; Weimin Liu; Xiaogang Liu; He Tian; Wei-Hong Zhu

doi:10.1038/s41467-020-14615-3

Nature Communications (Feb 2020)

De novo strategy with engineering anti-Kasha/Kasha fluorophores enables reliable ratiometric quantification of biomolecules

Limin Shi,
Chenxu Yan,
Zhiqian Guo,
Weijie Chi,
Jingle Wei,
Weimin Liu,
Xiaogang Liu,
He Tian,
Wei-Hong Zhu

Affiliations

Limin Shi: Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering, Feringa Nobel Prize Scientist Joint Research Center, Institute of Fine Chemicals, School of Chemistry and Molecular Engineering, East China University of Science and Technology
Chenxu Yan: Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering, Feringa Nobel Prize Scientist Joint Research Center, Institute of Fine Chemicals, School of Chemistry and Molecular Engineering, East China University of Science and Technology
Zhiqian Guo: Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering, Feringa Nobel Prize Scientist Joint Research Center, Institute of Fine Chemicals, School of Chemistry and Molecular Engineering, East China University of Science and Technology
Weijie Chi: Science and Math Cluster, Singapore University of Technology and Design
Jingle Wei: School of Physical Science and Technology, ShanghaiTech University
Weimin Liu: School of Physical Science and Technology, ShanghaiTech University
Xiaogang Liu: Science and Math Cluster, Singapore University of Technology and Design
He Tian: Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering, Feringa Nobel Prize Scientist Joint Research Center, Institute of Fine Chemicals, School of Chemistry and Molecular Engineering, East China University of Science and Technology
Wei-Hong Zhu: Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering, Feringa Nobel Prize Scientist Joint Research Center, Institute of Fine Chemicals, School of Chemistry and Molecular Engineering, East China University of Science and Technology

DOI: https://doi.org/10.1038/s41467-020-14615-3
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 11

Abstract

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Fluorescent probes are used in a number of fields but suffer from a lack of quantifiable results due to environmental effects. Here, the authors report on a dual-emission probe which can be used to detect the amount of probe present and the emission from detection applications to allow for quantification.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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