Spectro-temporal encoded multiphoton microscopy and fluorescence lifetime imaging at kilohertz frame-rates

Sebastian Karpf; Carson T. Riche; Dino Di Carlo; Anubhuti Goel; William A. Zeiger; Anand Suresh; Carlos Portera-Cailliau; Bahram Jalali

doi:10.1038/s41467-020-15618-w

Nature Communications (Apr 2020)

Spectro-temporal encoded multiphoton microscopy and fluorescence lifetime imaging at kilohertz frame-rates

Sebastian Karpf,
Carson T. Riche,
Dino Di Carlo,
Anubhuti Goel,
William A. Zeiger,
Anand Suresh,
Carlos Portera-Cailliau,
Bahram Jalali

Affiliations

Sebastian Karpf: Department of Electrical Engineering and Computational Science, University of California, Los Angeles (UCLA)
Carson T. Riche: Department of Bioengineering, University of California, Los Angeles (UCLA)
Dino Di Carlo: Department of Bioengineering, University of California, Los Angeles (UCLA)
Anubhuti Goel: Department of Neurology, University of California, Los Angeles (UCLA)
William A. Zeiger: Department of Neurology, University of California, Los Angeles (UCLA)
Anand Suresh: Department of Neurology, University of California, Los Angeles (UCLA)
Carlos Portera-Cailliau: Department of Neurology, University of California, Los Angeles (UCLA)
Bahram Jalali: Department of Electrical Engineering and Computational Science, University of California, Los Angeles (UCLA)

DOI: https://doi.org/10.1038/s41467-020-15618-w
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 9

Abstract

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Two-photon microscopy has been limited by low acquisition rates due to mechanical scanners. Here, the authors employ pulse-modulated, rapidly wavelength-swept lasers and inertia-free beam steering through angular dispersion in order to achieve non-linear microscopy with kilohertz frame rates.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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