Nature Communications (Nov 2020)
Enhancer hijacking determines extrachromosomal circular MYCN amplicon architecture in neuroblastoma
- Konstantin Helmsauer,
- Maria E. Valieva,
- Salaheddine Ali,
- Rocío Chamorro González,
- Robert Schöpflin,
- Claudia Röefzaad,
- Yi Bei,
- Heathcliff Dorado Garcia,
- Elias Rodriguez-Fos,
- Montserrat Puiggròs,
- Katharina Kasack,
- Kerstin Haase,
- Csilla Keskeny,
- Celine Y. Chen,
- Luis P. Kuschel,
- Philipp Euskirchen,
- Verena Heinrich,
- Michael I. Robson,
- Carolina Rosswog,
- Joern Toedling,
- Annabell Szymansky,
- Falk Hertwig,
- Matthias Fischer,
- David Torrents,
- Angelika Eggert,
- Johannes H. Schulte,
- Stefan Mundlos,
- Anton G. Henssen,
- Richard P. Koche
Affiliations
- Konstantin Helmsauer
- Department of Pediatric Oncology/Hematology, Charité—Universitätsmedizin Berlin
- Maria E. Valieva
- RG Development & Disease, Max Planck Institute for Molecular Genetics
- Salaheddine Ali
- RG Development & Disease, Max Planck Institute for Molecular Genetics
- Rocío Chamorro González
- Department of Pediatric Oncology/Hematology, Charité—Universitätsmedizin Berlin
- Robert Schöpflin
- RG Development & Disease, Max Planck Institute for Molecular Genetics
- Claudia Röefzaad
- Experimental and Clinical Research Center (ECRC), Max Delbrück Center for Molecular Medicine and Charité—Universitätsmedizin Berlin
- Yi Bei
- Department of Pediatric Oncology/Hematology, Charité—Universitätsmedizin Berlin
- Heathcliff Dorado Garcia
- Department of Pediatric Oncology/Hematology, Charité—Universitätsmedizin Berlin
- Elias Rodriguez-Fos
- Barcelona Supercomputing Center, Joint BSC-CRG-IRB Research Program in Computational Biology
- Montserrat Puiggròs
- Barcelona Supercomputing Center, Joint BSC-CRG-IRB Research Program in Computational Biology
- Katharina Kasack
- German Cancer Consortium (DKTK), partner site Berlin, and German Cancer Research Center DKFZ
- Kerstin Haase
- Department of Pediatric Oncology/Hematology, Charité—Universitätsmedizin Berlin
- Csilla Keskeny
- Department of Pediatric Oncology/Hematology, Charité—Universitätsmedizin Berlin
- Celine Y. Chen
- Department of Pediatric Oncology/Hematology, Charité—Universitätsmedizin Berlin
- Luis P. Kuschel
- Department of Neurology with Experimental Neurology, Charité—Universitätsmedizin Berlin
- Philipp Euskirchen
- German Cancer Consortium (DKTK), partner site Berlin, and German Cancer Research Center DKFZ
- Verena Heinrich
- Department of Computational Molecular Biology, Max Planck Institute for Molecular Genetics
- Michael I. Robson
- RG Development & Disease, Max Planck Institute for Molecular Genetics
- Carolina Rosswog
- Department of Experimental Pediatric Oncology, University Children’s Hospital of Cologne and Center for Molecular Medicine Cologne (CMMC), University of Cologne
- Joern Toedling
- Department of Pediatric Oncology/Hematology, Charité—Universitätsmedizin Berlin
- Annabell Szymansky
- Department of Pediatric Oncology/Hematology, Charité—Universitätsmedizin Berlin
- Falk Hertwig
- Department of Pediatric Oncology/Hematology, Charité—Universitätsmedizin Berlin
- Matthias Fischer
- Department of Experimental Pediatric Oncology, University Children’s Hospital of Cologne and Center for Molecular Medicine Cologne (CMMC), University of Cologne
- David Torrents
- Barcelona Supercomputing Center, Joint BSC-CRG-IRB Research Program in Computational Biology
- Angelika Eggert
- Department of Pediatric Oncology/Hematology, Charité—Universitätsmedizin Berlin
- Johannes H. Schulte
- Department of Pediatric Oncology/Hematology, Charité—Universitätsmedizin Berlin
- Stefan Mundlos
- RG Development & Disease, Max Planck Institute for Molecular Genetics
- Anton G. Henssen
- Department of Pediatric Oncology/Hematology, Charité—Universitätsmedizin Berlin
- Richard P. Koche
- Berlin Institute of Health
- DOI
- https://doi.org/10.1038/s41467-020-19452-y
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 12
Abstract
MYCN amplification is common in neuroblastomas. Here the authors analyse the MYCN amplicon structure and its epigenetic regulation by integrating short- and longread genomic and epigenomic data and find two classes of MYCN amplicons in neuroblastomas, one driven by local enhancers and the other by hijacking of distal regulatory elements.
