Effects of dietary collagen cofactors and hydroxyproline on the growth performance, textural properties and collagen deposition in swim bladder of Nibea coibor based on orthogonal array analysis

Haoji Guo; Xin Liu; Mengdu Tian; Guoquan Liu; Yuying Yuan; Xiaokang Ye; Haoran Zhang; Lanfei Xiao; Shuqi Wang; Yucong Hong; Kaihui Sun; Fan Lin; Xiaobo Wen

Aquaculture Reports (Dec 2022)

Effects of dietary collagen cofactors and hydroxyproline on the growth performance, textural properties and collagen deposition in swim bladder of Nibea coibor based on orthogonal array analysis

Haoji Guo,
Xin Liu,
Mengdu Tian,
Guoquan Liu,
Yuying Yuan,
Xiaokang Ye,
Haoran Zhang,
Lanfei Xiao,
Shuqi Wang,
Yucong Hong,
Kaihui Sun,
Fan Lin,
Xiaobo Wen

Affiliations

Haoji Guo: Guangdong Provincial Key Laboratory of Marine Biotechnology, Institute of Marine sciences, Shantou University, Shantou 515063, China
Xin Liu: Guangdong Provincial Key Laboratory of Marine Biotechnology, Institute of Marine sciences, Shantou University, Shantou 515063, China
Mengdu Tian: Guangdong Provincial Key Laboratory of Marine Biotechnology, Institute of Marine sciences, Shantou University, Shantou 515063, China
Guoquan Liu: Guangdong Provincial Key Laboratory of Marine Biotechnology, Institute of Marine sciences, Shantou University, Shantou 515063, China
Yuying Yuan: Guangdong Provincial Key Laboratory of Marine Biotechnology, Institute of Marine sciences, Shantou University, Shantou 515063, China
Xiaokang Ye: Guangdong Provincial Key Laboratory of Marine Biotechnology, Institute of Marine sciences, Shantou University, Shantou 515063, China
Haoran Zhang: College of Marine Sciences, South China Agricultural University, Guangzhou 510642, China
Lanfei Xiao: College of Marine Sciences, South China Agricultural University, Guangzhou 510642, China
Shuqi Wang: Guangdong Provincial Key Laboratory of Marine Biotechnology, Institute of Marine sciences, Shantou University, Shantou 515063, China
Yucong Hong: Guangdong Yuequn Biotechnology Co., Ltd, Guangdong Provincial Key Laboratory of Aquatic Larvae Feed, Jieyang 522000, China
Kaihui Sun: Guangdong Yuequn Biotechnology Co., Ltd, Guangdong Provincial Key Laboratory of Aquatic Larvae Feed, Jieyang 522000, China
Fan Lin: Guangdong Provincial Key Laboratory of Marine Biotechnology, Institute of Marine sciences, Shantou University, Shantou 515063, China; Corresponding author.
Xiaobo Wen: Guangdong Provincial Key Laboratory of Marine Biotechnology, Institute of Marine sciences, Shantou University, Shantou 515063, China; College of Marine Sciences, South China Agricultural University, Guangzhou 510642, China; Corresponding author at: Guangdong Provincial Key Laboratory of Marine Biotechnology, Institute of Marine sciences, Shantou University, Shantou 515063, China.

Journal volume & issue: Vol. 27
p. 101375

Abstract

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The global demand for collagen is continuously growing over years. Nutritional regulation of quality and collagen deposition in the swim bladder, an ideal collagen source of marine product, is a promising method for improving the economic value of fish. In this study, to enhance growth efficiency and collagen deposition in swim bladder of an important economic marine fish, the chu’s croaker (Nibea coibor), a L9(34) orthogonal array was designed with three levels for each factor, which are hydroxyproline (Hyp) and the key cofactors for proline hydroxylation during collagen post-translation modification, such as A1-A3 (Hyp): 0, 10 and 20 g/kg; B1-B3 (vitamin C, VC): 0, 0.20 and 0.40 g/kg; C1-C3 (Fe2+): 0, 0.20 and 0.40 g/kg; D1-D3 (α-ketoglutarate, AKG): 0, 10 and 20 g/kg, to formulate the trial feeds. An 8-week feed trial was carried out on 405 fish (initial weight 269.04 ± 3.18 g) which were randomly distributed into the nine treatment groups with three replicates of 15 fish per cage. Results indicated that appropriate increase of dietary Hyp and VC level is more beneficial to collagen deposition and textural properties in swim bladder, while Hyp and AKG exerted more significant effects on the growth performance. Gene expression analysis of collagen metabolism indicated that Hyp mainly improved the synthesis of type I collagen and inhibited collagen degradation, while VC and AKG mainly play an important role in hydroxylation of proline residues on collagen. The best combination for growth performance and collagen deposition in swim bladder were suggested to be A2B2C1D3 and A2B2C1D1, respectively. The results demonstrated that appropriate dietary supplementation of Hyp and cofactors can improve growth and collagen production of swim bladder. Our result would provide reference data for nutritional regulation of the species and optimization of the commercial feed to provide fish products with high quality.

Published in Aquaculture Reports

ISSN: 2352-5134 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Agriculture: Aquaculture. Fisheries. Angling
Website: http://www.journals.elsevier.com/aquaculture-reports/

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