Changes of coagulation-fibrinolysis function after soft tissue injury complicated with shock and seawater immersion in rabbits

Abstract

Objective To observe and analyze the changes of coagulation and fibrinolytic function after soft tissue injured combined with shock and seawater immersion in rabbits. Methods Twenty rabbits of either gender were provided by the Laboratory Animal Center of the Army Medical University, with a body weight of 2.17±0.02 kg, and were randomly divided into the group A and the group B (n=10).The calf and thigh muscle of rabbits in the group A were injured with a total area of about 10 cm2 and a depth of about 1.5 cm.Then 20% of the total blood volume of rabbits was extracted to make the animal model for injured soft tissue complicated with hemorrhagic shock.Animals in group B were immersed in simulated seawater for 40 min on the basis of the procedure in the group A mentioned before, and the depth of immersion was at the lower margin of ribs.Before and after 15 min and 1, 4, and 8 h of getting injured, blood samples were taken to detect thromboelastography, routine coagulation phase, platelet count and D-dimer level, and the changes in blood pressure and survival rate were observed at each time point. Results The mean arterial pressure of rabbits in the 2 groups was decreased by 30% after injury.The model of rabbits with injured soft tissue combined with hemorrhagic shock was established successfully, and there was no significant difference in shock between the 2 groups.The changes of coagulation and fibrinolytic function in the group B were more complex and serious than those in the group A, which were manifested at 8 h after getting injury.The activation time of partial thrombin was significantly increased (29.53±4.36 vs 24.56±4.1 s), the reaction time was significantly increased (3.07±0.69 vs 2.34±0.63 min), the D-dimer concentration was significantly increased (0.214±0.23 vs 0.037±0.03 mg/L), platelet activation activity was significantly increased (508.67±86.69 vs 381.60±73.84), and the maximum amplitude was significantly lower (52.73±14.97 vs 63.52±3.86 mm).The platelet count in the group B was significantly lower than that in the group A[(182.77±60.01)×109/L vs(236.78±52.14)×109/L]. Conclusion In the case of injured soft tissue combined with shock, seawater immersion may result in longer coagulation time, higher fibrinolytic activation and obvious platelet reduction than those without seawater immersion.

Keywords

• soft tissue
• seawater immersion
• hemorrhagic shock
• coagulation-fibrinolysis system
• thrombelastography