A monomeric mycobacteriophage immunity repressor utilizes two domains to recognize an asymmetric DNA sequence

Reliza J. McGinnis; Chad A. Brambley; Brandon Stamey; William C. Green; Kimberly N. Gragg; Erin R. Cafferty; Thomas C. Terwilliger; Michal Hammel; Thomas J. Hollis; Justin M. Miller; Maria D. Gainey; Jamie R. Wallen

doi:10.1038/s41467-022-31678-6

Nature Communications (Jul 2022)

A monomeric mycobacteriophage immunity repressor utilizes two domains to recognize an asymmetric DNA sequence

Reliza J. McGinnis,
Chad A. Brambley,
Brandon Stamey,
William C. Green,
Kimberly N. Gragg,
Erin R. Cafferty,
Thomas C. Terwilliger,
Michal Hammel,
Thomas J. Hollis,
Justin M. Miller,
Maria D. Gainey,
Jamie R. Wallen

Affiliations

Reliza J. McGinnis: Western Carolina University, Department of Chemistry and Physics
Chad A. Brambley: Middle Tennessee State University, Department of Chemistry
Brandon Stamey: Western Carolina University, Department of Chemistry and Physics
William C. Green: Western Carolina University, Department of Chemistry and Physics
Kimberly N. Gragg: Western Carolina University, Department of Chemistry and Physics
Erin R. Cafferty: Western Carolina University, Department of Chemistry and Physics
Thomas C. Terwilliger: New Mexico Consortium
Michal Hammel: Molecular Biophysics and Integrated Bioimaging, Lawrence Berkeley National Laboratory
Thomas J. Hollis: Department of Biochemistry, Wake Forest University School of Medicine, Medical Center Boulevard
Justin M. Miller: Middle Tennessee State University, Department of Chemistry
Maria D. Gainey: Western Carolina University, Department of Chemistry and Physics
Jamie R. Wallen: Western Carolina University, Department of Chemistry and Physics

DOI: https://doi.org/10.1038/s41467-022-31678-6
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 15

Abstract

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Bacteriophage repressor proteins downregulate viral lytic gene expression. Herein, the authors present the X-ray crystal structure of a monomeric repressor that binds an asymmetric DNA sequence using two independent domains.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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