Prime editing efficiency and fidelity are enhanced in the absence of mismatch repair

J. Ferreira da Silva; G. P. Oliveira; E. A. Arasa-Verge; C. Kagiou; A. Moretton; G. Timelthaler; J. Jiricny; J. I. Loizou

doi:10.1038/s41467-022-28442-1

Nature Communications (Feb 2022)

Prime editing efficiency and fidelity are enhanced in the absence of mismatch repair

J. Ferreira da Silva,
G. P. Oliveira,
E. A. Arasa-Verge,
C. Kagiou,
A. Moretton,
G. Timelthaler,
J. Jiricny,
J. I. Loizou

Affiliations

J. Ferreira da Silva: Institute of Cancer Research, Department of Medicine I, Comprehensive Cancer Centre, Medical University of Vienna
G. P. Oliveira: Institute of Cancer Research, Department of Medicine I, Comprehensive Cancer Centre, Medical University of Vienna
E. A. Arasa-Verge: Institute of Cancer Research, Department of Medicine I, Comprehensive Cancer Centre, Medical University of Vienna
C. Kagiou: Institute of Cancer Research, Department of Medicine I, Comprehensive Cancer Centre, Medical University of Vienna
A. Moretton: Institute of Cancer Research, Department of Medicine I, Comprehensive Cancer Centre, Medical University of Vienna
G. Timelthaler: Institute of Cancer Research, Department of Medicine I, Comprehensive Cancer Centre, Medical University of Vienna
J. Jiricny: Institute of Biochemistry of the ETH Zurich
J. I. Loizou: Institute of Cancer Research, Department of Medicine I, Comprehensive Cancer Centre, Medical University of Vienna

DOI: https://doi.org/10.1038/s41467-022-28442-1
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 11

Abstract

Read online

Prime Editing is a versatile genome engineering tool. Here, the authors identify the DNA repair pathway known as mismatch repair as inhibitory for Prime Editing, thus, loss of mismatch repair enhances the efficiency of Prime Editing.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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