Nature Communications (Jul 2022)

Cross-validation of distance measurements in proteins by PELDOR/DEER and single-molecule FRET

  • Martin F. Peter,
  • Christian Gebhardt,
  • Rebecca Mächtel,
  • Gabriel G. Moya Muñoz,
  • Janin Glaenzer,
  • Alessandra Narducci,
  • Gavin H. Thomas,
  • Thorben Cordes,
  • Gregor Hagelueken

DOI
https://doi.org/10.1038/s41467-022-31945-6
Journal volume & issue
Vol. 13, no. 1
pp. 1 – 19

Abstract

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Pulsed electron-electron double resonance spectroscopy (PELDOR/DEER) and single-molecule Förster resonance energy transfer spectroscopy (smFRET) are used to determine conformational changes and probe distances in biological macromolecules. Here the authors compare the methods on a large set of samples.