Essential Oils from Leaves of <i>Juniperus thurifera</i> L., Exhibiting Antioxidant, Antifungal and Antibacterial Activities against Antibiotic-Resistant Microbes

Soufyane Lafraxo; Azeddin El Barnossi; Abdelfattah El Moussaoui; Mohammed Bourhia; Ahmad Mohammad Salamatullah; Abdulhakeem Alzahrani; Aziz Ait Akka; Abderahmane Choubbane; Mohamed Akhazzane; Mourad A. M. Aboul-Soud; John P. Giesy; Amina Bari

doi:10.3390/horticulturae8040321

Horticulturae (Apr 2022)

Essential Oils from Leaves of <i>Juniperus thurifera</i> L., Exhibiting Antioxidant, Antifungal and Antibacterial Activities against Antibiotic-Resistant Microbes

Soufyane Lafraxo,
Azeddin El Barnossi,
Abdelfattah El Moussaoui,
Mohammed Bourhia,
Ahmad Mohammad Salamatullah,
Abdulhakeem Alzahrani,
Aziz Ait Akka,
Abderahmane Choubbane,
Mohamed Akhazzane,
Mourad A. M. Aboul-Soud,
John P. Giesy,
Amina Bari

Affiliations

Soufyane Lafraxo: Laboratory of Biotechnology, Environment, Agri-Food and Health, Faculty of Sciences Dhar El Mahraz, Sidi Mohammed Ben Abdellah University, P.O. Box 1796 (Atlas), Fez 30000, Morocco
Azeddin El Barnossi: Laboratory of Biotechnology, Environment, Agri-Food and Health, Faculty of Sciences Dhar El Mahraz, Sidi Mohammed Ben Abdellah University, P.O. Box 1796 (Atlas), Fez 30000, Morocco
Abdelfattah El Moussaoui: Laboratory of Biotechnology, Environment, Agri-Food and Health, Faculty of Sciences Dhar El Mahraz, Sidi Mohammed Ben Abdellah University, P.O. Box 1796 (Atlas), Fez 30000, Morocco
Mohammed Bourhia: Laboratory of Chemistry, Biochemistry, Nutrition, and Environment, Faculty of Medicine and Pharmacy, University Hassan II, Casablanca 20000, Morocco
Ahmad Mohammad Salamatullah: Department of Food Science & Nutrition, College of Food and Agricultural Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi Arabia
Abdulhakeem Alzahrani: Department of Food Science & Nutrition, College of Food and Agricultural Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi Arabia
Aziz Ait Akka: Laboratory of Biotechnology, Conservation and Valorisation of Natural Resources, Faculty of Sciences Dhar El Mahraz, Sidi Mohammed Ben Abdallah University, P.O. Box 1796 (Atlas), Fez 30000, Morocco
Abderahmane Choubbane: Laboratory of Biotechnology, Environment, Agri-Food and Health, Faculty of Sciences Dhar El Mahraz, Sidi Mohammed Ben Abdellah University, P.O. Box 1796 (Atlas), Fez 30000, Morocco
Mohamed Akhazzane: Engineering Laboratory of Organometallic and Molecular Materials and Environment, Faculty of Sciences Dhar El Mahraz, Sidi Mohamed Ben Abdellah University, P.O. Box 1796 (Atlas), Fez 30000, Morocco
Mourad A. M. Aboul-Soud: Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, P.O. Box 10219, Riyadh 11433, Saudi Arabia
John P. Giesy: Toxicology Centre, University of Saskatchewan, Saskatoon, SK S7N 5B3, Canada
Amina Bari: Laboratory of Biotechnology, Environment, Agri-Food and Health, Faculty of Sciences Dhar El Mahraz, Sidi Mohammed Ben Abdellah University, P.O. Box 1796 (Atlas), Fez 30000, Morocco

DOI: https://doi.org/10.3390/horticulturae8040321
Journal volume & issue: Vol. 8, no. 4
p. 321

Abstract

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The antioxidant, antibacterial and antifungal properties of essential oils (EOs) of Juniperus thurifera L., a plant utilized in traditional, herbal medicine, were investigated. The EOs were extracted by use of a Clevenger apparatus and phytochemicals identified by gas chromatography coupled with mass spectrometry (GC/MS/MS). The antioxidant capacity of EOs of J. thurifera was determined by 2,2-diphenyl-1-picrylhydrazil (DPPH), total antioxidant capacity (TAC), and ferric reducing antioxidant power (FRAP). Antimicrobial activity of EOs of J. thurifera was determined against four fungal strains, Candida albicans; ATCC 10231, Aspergillus niger; MTCC 282, Aspergillus flavus; MTCC 9606 and Fusarium oxysporum; MTCC 9913 and four bacterial strains, Staphylococcus aureus; ATCC 6633, Escherichia coli; K12, Bacillus subtilis; DSM 6333, and Pseudomonas aeruginosa; CIP A22, by use of the disk diffusion method, and microdilution method used to determine the minimum inhibitory concentration (MIC). EOs of J. thurifera consisted of 31 compounds and were dominated by α-thujene (25%), elemol (12%) and muurolol (12%). Antioxidant activity recorded an IC50 of 24 ± 0.71 µg/mL (DPPF), EC50 of 0.19 ± 0.01 mg/mL (FRAP), and 9.3 × 102 ± 38 mg EAA/g (TAC). The EOs of J. thurifera exhibited significant antibacterial activity against all bacterial strains under investigation, especially P. aeruginosa; CIP A22 with an inhibition diameter of 28 ± 1.5 mm and MIC of 4.8 × 10−2 ± 0. 001 µg/mL. EOs of J. thurifera also exhibited significant antifungal activity against C. albicans; ATCC 10231 and F. oxysporum; MTCC 9913 with an activity of 21 ± 2.1 mm, 32 ± 2.3%, and MIC of 9.5 × 10−2 ± 0.001 Bioactive molecules found in EOs of J. thurifera could be used as an alternative solution to antibiotics available on the market to combat microbial resistance.

Published in Horticulturae

ISSN: 2311-7524 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Agriculture: Plant culture
Website: http://www.mdpi.com/journal/horticulturae

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