Agricultural & Environmental Letters (Dec 2016)

Cryptosporidium Soil Extraction by Filtration/IMS/FA Compatible with USEPA Method 1623.1

  • Zachariah P. Zopp,
  • Jeremy M. Olstadt,
  • K. G. Karthikeyan,
  • Anita M. Thompson,
  • Sharon C. Long

DOI
https://doi.org/10.2134/ael2016.08.0031
Journal volume & issue
Vol. 1, no. 1

Abstract

Read online

a parasitic pathogen, when present in drinking water supplies poses a significant risk to human health. The soil environment provides a critical linkage between parent material (bovine manure) and the surface and groundwaters used by susceptible human populations for drinking water. The lack of a USEPA published analysis for the determination of in soil is a barrier to standardized investigation of transport in soils. Methodologies have been published in the absence of a USEPA analysis, yet they lack sufficient detail and quality controls to be generally applicable. This study developed and evaluated a soil extraction method capable of producing an extracted fluid containing that could be purified and enumerated using USEPA Method 1623.1.