Compare the effectiveness of extracorporeal shockwave and hyperbaric oxygen therapy on enhancing wound healing in a streptozotocin‐induced diabetic rodent model

Abstract

Abstract Studies have revealed that both extracorporeal shock‐wave therapy (ESWT) and hyperbaric oxygen therapy (HBOT) can accelerate wound healing. This study aimed to compare the effectiveness of ESWT and HBOT in enhancing diabetic wound healing. A dorsal skin defect in a streptozotocin‐induced diabetes rodent model was used. Postoperative wound healing was assessed once every 3 days. Histologic examination was performed with hematoxylin and eosin staining. Proliferation marker protein Ki‐67 (Ki‐67), endothelial nitric oxide synthase (eNOS), vascular endothelial growth factor (VEGF), and 8‐hydroxy‐2‐deoxyguanosine (8‐OHdG) were evaluated with immunohistochemical (IHC) staining. The wound area was significantly reduced in the ESWT and HBOT groups compared to that in the diabetic controls. However, the wound healing time was significantly increased in the HBOT group compared to the ESWT group. Histological findings showed a statistical increase in neovascularization and suppression of the inflammatory response by both HBOT and ESWT compared to the controls. IHC staining revealed a significant increase in Ki‐67, VEGF, and eNOS but suppressed 8‐OHdG expression in the ESWT group compared to the HBOT group. ESWT facilitated diabetic wound healing more effectively than HBOT by suppressing the inflammatory response and enhancing cellular proliferation and neovascularization and tissue regeneration.

Keywords

• diabetic foot ulcers
• diabetic wound healing
• extracorporeal shock‐wave therapy
• hyperbaric oxygen therapy
• streptozotocin