Nature Communications (Apr 2019)
Dishevelled-3 conformation dynamics analyzed by FRET-based biosensors reveals a key role of casein kinase 1
- Jakub Harnoš,
- Maria Consuelo Alonso Cañizal,
- Miroslav Jurásek,
- Jitender Kumar,
- Cornelia Holler,
- Alexandra Schambony,
- Kateřina Hanáková,
- Ondřej Bernatík,
- Zbyněk Zdráhal,
- Kristína Gömöryová,
- Tomáš Gybeľ,
- Tomasz Witold Radaszkiewicz,
- Marek Kravec,
- Lukáš Trantírek,
- Jan Ryneš,
- Zankruti Dave,
- Ana Iris Fernández-Llamazares,
- Robert Vácha,
- Konstantinos Tripsianes,
- Carsten Hoffmann,
- Vítězslav Bryja
Affiliations
- Jakub Harnoš
- Department of Experimental Biology, Faculty of Science, Masaryk University
- Maria Consuelo Alonso Cañizal
- Department of Pharmacology and Toxicology, University of Würzburg
- Miroslav Jurásek
- CEITEC—Central European Institute of Technology, Masaryk University
- Jitender Kumar
- CEITEC—Central European Institute of Technology, Masaryk University
- Cornelia Holler
- Max Planck Institute for the Science of Light
- Alexandra Schambony
- Max Planck Institute for the Science of Light
- Kateřina Hanáková
- CEITEC—Central European Institute of Technology, Masaryk University
- Ondřej Bernatík
- Department of Experimental Biology, Faculty of Science, Masaryk University
- Zbyněk Zdráhal
- CEITEC—Central European Institute of Technology, Masaryk University
- Kristína Gömöryová
- Department of Experimental Biology, Faculty of Science, Masaryk University
- Tomáš Gybeľ
- Department of Experimental Biology, Faculty of Science, Masaryk University
- Tomasz Witold Radaszkiewicz
- Department of Experimental Biology, Faculty of Science, Masaryk University
- Marek Kravec
- Department of Experimental Biology, Faculty of Science, Masaryk University
- Lukáš Trantírek
- CEITEC—Central European Institute of Technology, Masaryk University
- Jan Ryneš
- CEITEC—Central European Institute of Technology, Masaryk University
- Zankruti Dave
- Department of Experimental Biology, Faculty of Science, Masaryk University
- Ana Iris Fernández-Llamazares
- Pepscan Therapeutics B.V.
- Robert Vácha
- CEITEC—Central European Institute of Technology, Masaryk University
- Konstantinos Tripsianes
- CEITEC—Central European Institute of Technology, Masaryk University
- Carsten Hoffmann
- Department of Pharmacology and Toxicology, University of Würzburg
- Vítězslav Bryja
- Department of Experimental Biology, Faculty of Science, Masaryk University
- DOI
- https://doi.org/10.1038/s41467-019-09651-7
- Journal volume & issue
-
Vol. 10,
no. 1
pp. 1 – 18
Abstract
Dishevelled (DVL) is the key component of Wnt signaling pathway that acts as a signaling hub. Here, authors study the conformational dynamics of DVL3 in vivo using their engineered FlAsH-based FRET biosensors and describe how Wnt activation opens DVL and facilitates Frizzled recruitment.
