Generation of human induced pluripotent stem cells (SDUKIi002-A) from a 22-year-old male diagnosed with autism spectrum disorder

Morad Kamand; Mirolyuba Ilieva; Sheena Louise Forsberg; Mads Thomassen; Åsa Fex Svenningsen; Bjørn Holst; Morten Meyer; Tanja Maria Michel

Stem Cell Research (Jul 2020)

Generation of human induced pluripotent stem cells (SDUKIi002-A) from a 22-year-old male diagnosed with autism spectrum disorder

Morad Kamand,
Mirolyuba Ilieva,
Sheena Louise Forsberg,
Mads Thomassen,
Åsa Fex Svenningsen,
Bjørn Holst,
Morten Meyer,
Tanja Maria Michel

Affiliations

Morad Kamand: Institute of Clinical Research, Department of Psychiatry Research, University of Southern Denmark, Odense, Denmark; Institute of Molecular Medicine, Department of Neurobiology Research, University of Southern Denmark, Odense, Denmark; Psychiatry in the Region of Southern Denmark, Odense University Hospital, Denmark
Mirolyuba Ilieva: Institute of Clinical Research, Department of Psychiatry Research, University of Southern Denmark, Odense, Denmark; Psychiatry in the Region of Southern Denmark, Odense University Hospital, Denmark
Sheena Louise Forsberg: Institute of Clinical Research, Department of Psychiatry Research, University of Southern Denmark, Odense, Denmark; Psychiatry in the Region of Southern Denmark, Odense University Hospital, Denmark
Mads Thomassen: Institute of Clinical Research, Department of Human Genetics, Odense University Hospital, Denmark
Åsa Fex Svenningsen: Institute of Molecular Medicine, Department of Neurobiology Research, University of Southern Denmark, Odense, Denmark; Brain Research – Inter Disciplinary Guided Excellence (BRIDGE), Odense, Denmark
Bjørn Holst: Bioneer A/S, Hørsholm, Denmark
Morten Meyer: Institute of Molecular Medicine, Department of Neurobiology Research, University of Southern Denmark, Odense, Denmark; Brain Research – Inter Disciplinary Guided Excellence (BRIDGE), Odense, Denmark; Department of Neurology, Odense University Hospital, Denmark
Tanja Maria Michel: Institute of Clinical Research, Department of Psychiatry Research, University of Southern Denmark, Odense, Denmark; Brain Research – Inter Disciplinary Guided Excellence (BRIDGE), Odense, Denmark; Psychiatry in the Region of Southern Denmark, Odense University Hospital, Denmark; Corresponding author at: Institute of Clinical Research, Department of Psychiatry Research, University of Southern Denmark, Odense, Denmark.

Journal volume & issue: Vol. 46
p. 101834

Abstract

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Autism spectrum disorders are characterized by impaired social interaction and communication as well as restricted and repetitive interests and behavior. Increasing evidence points to an early-stage disruption of brain development. A human-induced pluripotent stem cell line (SDUKIi002-A) was created from skin fibroblasts from a 22-year old autistic male identified in the “FYNEN-cohort” of Southern Denmark. Reprogramming of the fibroblasts was performed using integration-free episomal plasmids. Further characterization confirmed the expression of pluripotency markers, differentiation into the three germ layers, absence of chromosomal abnormalities, and mycoplasma infection.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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