PLoS ONE (Jan 2014)

Whole genomic sequence and replication kinetics of a new enterovirus C96 isolated from Guangdong, China with a different cell tropism.

  • Jing Lu,
  • Huanying Zheng,
  • Yong Zhang,
  • Xue Guo,
  • De Wu,
  • Hui Li,
  • Leng Liu,
  • Hanri Zeng,
  • Lina Yi,
  • Ling Fang,
  • Yanling Mo,
  • Wenbo Xu,
  • Changwen Ke

DOI
https://doi.org/10.1371/journal.pone.0086877
Journal volume & issue
Vol. 9, no. 1
p. e86877

Abstract

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Enterovirus 96 (EV-C96) is a newly described serotype within the enterovirus C (EV-C) species, and its biological and pathological characters are largely unknown. In this study, we sequenced the whole genome of a novel EV-C96 strain that was isolated in 2011 from a patient with acute flaccid paralysis (AFP) in Guangdong province, China and characterized the properties of its infection. Sequence analysis revealed the close relationship between the EV-C96 strains isolated from the Guangdong and Shandong provinces of China, and suggested that recombination events occurred both between these EV-C96 strains and with other EV-C viruses. Moreover, the virus replication kinetics showed EV-C96 Guangdong strain replicated at a high rate in RD cells and presented a different cell tropism to other strains isolated from Shandong recently. These findings gave further insight into the evolutionary processes and extensive biodiversity of EV-C96.