Quantitative Measurements of HIV-1 and Dextran Capture by Human Monocyte-derived Dendritic Cells (MDDCs)

Mickaël Ménager; Dan  Littman

doi:10.21769/BioProtoc.2004

Bio-Protocol (Nov 2016)

Quantitative Measurements of HIV-1 and Dextran Capture by Human Monocyte-derived Dendritic Cells (MDDCs)

Mickaël Ménager,
Dan Littman

Affiliations

Mickaël Ménager: The Kimmel Center for Biology and Medicine of the Skirball Institute, New York University School of Medicine, New York, USA
Dan Littman: The Kimmel Center for Biology and Medicine of the Skirball Institute, New York University School of Medicine, New York, USAHoward Hughes Medical Institute, Washington, USA

DOI: https://doi.org/10.21769/BioProtoc.2004
Journal volume & issue: Vol. 6, no. 22

Abstract

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The aim of this protocol is to describe how to measure and quantify the amount of HIV-1 particles and dextran molecules internalized in human monocyte derived dendritic cells (MDDCs), using three different techniques: flow cytometry, quantitative PCR and confocal microscopy.

Published in Bio-Protocol

ISSN: 2331-8325 (Online)
Publisher: Bio-protocol LLC
Country of publisher: United States
LCC subjects: Science: Biology (General)
Website: https://bio-protocol.org

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