Development of Three-Dimensional Micro Vibration Stage and Its Application to Control Device for Cell Culture

Abstract

In this study, a new three-dimensional micro vibration stage is developed, in which a doubly steric L-shaped clamped-free beam type vibrator is utilized. The developed three-dimensional micro vibration stage, which has very simple structure and is quite easily sterilized, is extended to control system of cell culture. Focusing on osteoblast character which is sensitive to external mechanical stimuli, a normal human osteoblast is chosen as a test subject. Dynamic stimulation is applied to the normal human osteoblast utilizing the developed three-dimensional vibration stage. In order to estimate effect of dynamic stimulation upon cultured cell, we define parameters of projected area and slenderness ratio of the cultured normal human osteoblast. Statically cultured cells of 4th and 6th passage were provided for experiments. It was observed that the projected area of normal human osteoblast increased, while the slenderness ratio decreased according to the number of cell passage increasing. Shape of the 6th passage of dynamically stimulated cells was not similar to that of same passage of control, but to that of 4th passage of control. From the viewpoint of morphology, the present study has shown the significant effect of dynamic stimulation upon cultured cells.

Keywords

• three dimensional vibration stage
• piezoelectric vibrator
• three dimensional stimulation
• normal human osteoblast
• culture control
• actin filament