DNA requirement in FANCD2 deubiquitination by USP1-UAF1-RAD51AP1 in the Fanconi anemia DNA damage response

Fengshan Liang; Adam S. Miller; Simonne Longerich; Caroline Tang; David Maranon; Elizabeth A. Williamson; Robert Hromas; Claudia Wiese; Gary M. Kupfer; Patrick Sung

doi:10.1038/s41467-019-10408-5

Nature Communications (Jun 2019)

DNA requirement in FANCD2 deubiquitination by USP1-UAF1-RAD51AP1 in the Fanconi anemia DNA damage response

Fengshan Liang,
Adam S. Miller,
Simonne Longerich,
Caroline Tang,
David Maranon,
Elizabeth A. Williamson,
Robert Hromas,
Claudia Wiese,
Gary M. Kupfer,
Patrick Sung

Affiliations

Fengshan Liang: Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine
Adam S. Miller: Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine
Simonne Longerich: Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine
Caroline Tang: Department of Pediatrics, Section of Hematology-Oncology, Yale University School of Medicine
David Maranon: Department of Environmental and Radiological Health Sciences, Colorado State University
Elizabeth A. Williamson: Department of Medicine, University of Texas Health Science Center at San Antonio
Robert Hromas: Department of Medicine, University of Texas Health Science Center at San Antonio
Claudia Wiese: Department of Environmental and Radiological Health Sciences, Colorado State University
Gary M. Kupfer: Department of Pediatrics, Section of Hematology-Oncology, Yale University School of Medicine
Patrick Sung: Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine

DOI: https://doi.org/10.1038/s41467-019-10408-5
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 8

Abstract

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In the Fanconi anemia pathway, deubiquitination of FANCD2 is a fundamental regulatory step. Here, the authors have developed a set of biochemical tools to reconstitute FANCD2 deubiquitination by recombinant USP1-UAF1-RAD51AP1 and reveal critical mechanistic details of the process.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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