Ciência Rural ()

Strategies for exchange of coconut germplasm in Brazil

  • Ana da Silva Lédo,
  • Leila Albuquerque Resende de Oliveira,
  • Caroline de Araújo Machado,
  • Ana Veruska Cruz da Silva,
  • Semíramis Rabelo Ramalho Ramos

DOI
https://doi.org/10.1590/0103-8478cr20160391
Journal volume & issue
Vol. 47, no. 3

Abstract

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ABSTRACT: Seed size and its physiology are a barrier for the exchange of coconut genetics resources and the tissue culture techniques can assist the germplasm bank enrichment. The aim of this study was to evaluate the effect of transport and storage procedures of zygotic embryos to promote the exchange of coconut accessions. Mature zygotic embryo from Cameroon red dwarf (CRD), Malayan yellow dwarf (MYD) and Malayan red dwarf (MRD) were used. The following treatments were evaluated: T1-storage of endosperm disc in plastic bags at 10±2°C for 5 days; T2 - 8 days; T3 - 12 days; T4 - embryo excised and inoculated in micro tube with Y3 culture medium with no sucrose for 2 days and T5 - five embryos inoculated in Y3 culture medium without sucrose in Petri dish for 2 days. T5 treatment resulted in low bacterial contamination.

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