Visible light photoacoustic ophthalmoscopy and near-infrared-II optical coherence tomography in the mouse eye

R. Haindl; V. Bellemo; P. Rajendran; B. Tan; M. Liu; B. S. Lee; Q. Zhou; R. A. Leitgeb; W. Drexler; L. Schmetterer; M. Pramanik

doi:10.1063/5.0168091

APL Photonics (Oct 2023)

Visible light photoacoustic ophthalmoscopy and near-infrared-II optical coherence tomography in the mouse eye

R. Haindl,
V. Bellemo,
P. Rajendran,
B. Tan,
M. Liu,
B. S. Lee,
Q. Zhou,
R. A. Leitgeb,
W. Drexler,
L. Schmetterer,
M. Pramanik

Affiliations

R. Haindl: School of Chemistry, Chemical Engineering and Biotechnology, Nanyang Technological University, Singapore
V. Bellemo: Singapore Eye Research Institute, Singapore National Eye Centre, Singapore
P. Rajendran: Department of Radiation Oncology, Stanford University, Stanford, California 94305, USA
B. Tan: Singapore Eye Research Institute, Singapore National Eye Centre, Singapore
M. Liu: Centre for Medical Physics and Biomedical Engineering, Medical University of Vienna, Vienna, Austria
B. S. Lee: Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore
Q. Zhou: Department of Biomedical Engineering, University of Southern California, Los Angeles, California 90089, USA
R. A. Leitgeb: Centre for Medical Physics and Biomedical Engineering, Medical University of Vienna, Vienna, Austria
W. Drexler: Centre for Medical Physics and Biomedical Engineering, Medical University of Vienna, Vienna, Austria
L. Schmetterer: School of Chemistry, Chemical Engineering and Biotechnology, Nanyang Technological University, Singapore
M. Pramanik: Department of Electrical and Computer Engineering, Iowa State University, Ames, Iowa 50011, USA

DOI: https://doi.org/10.1063/5.0168091
Journal volume & issue: Vol. 8, no. 10
pp. 106108 – 106108-11

Abstract

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Non-invasive imaging plays a crucial role in diagnosing and studying eye diseases. However, existing photoacoustic ophthalmoscopy (PAOM) techniques in mice have limitations due to handling restrictions, suboptimal optical properties, limited availability of light sources, and permissible light fluence at the retina. This study introduces an innovative approach that utilizes Rose Bengal, a contrast agent, to enhance PAOM contrast. This enables visualization of deeper structures, such as the choroidal vasculature and sclera in the mouse eye, using visible light. The integration of near-infrared-II (NIR-II) optical coherence tomography provides additional tissue contrast and insights into potential NIR-II PAOM capabilities. To optimize imaging, we developed a cost-effective 3D printable mouse eye phantom and a fully 3D printable tip/tilt mouse platform. This solution elevates PAOM to a user-friendly technology, which can be used to address pressing research questions concerning several ocular diseases, such as myopia, glaucoma, and/or age-related macular degeneration in the future.

Published in APL Photonics

ISSN: 2378-0967 (Online)
Publisher: AIP Publishing LLC
Country of publisher: United States
LCC subjects: Technology: Engineering (General). Civil engineering (General): Applied optics. Photonics
Website: https://aplphotonics.aip.org

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