Identification of 18 polymorphic microsatellite loci in the spruce bark beetle Ips typographus (Coleoptera: Scolytidae) using high-throughput sequence data

Abstract

Novel microsatellite markers for the spruce bark beetle (Ips typographus L.) are identified and characterized using next generation sequencing technology. 18 polymorphic loci were obtained by screening 10,684 reads and tested on 35 bark beetle samples from different locations in Bavaria, Germany. Allelic richness ranged from two to 38 alleles, observed heterozygosity from 0.03 to 0.66 and expected heterozygosity from 0.08 to 0.97. Four loci showed significant deviation from the Hardy-Weinberg equilibrium and no linkage between loci was detected. The 18 loci, along with another six loci previously described, provide effective analytical tools for analyzing the fine-scale genetic structure of bark beetle populations. The result of this study demonstrates that next generation sequencing technology is a valuable method for isolating microsatellites of a coleopteran species.

Keywords

• coleoptera
• curculionidae
• ips typographus
• 454 sequencing
• next generation sequencing
• short tandem repeats