Quantitative analysis of individual bile acids by gas-liquid chromatography: an improved method

Abstract

The quantitative analysis of individual bile acids by gas-liquid chromatography has been improved by column oven temperature programming and by a new liquid phase, SP-2401. The method is fast; bile acids are well resolved; retention times are reproducible; detector responses are linear and sensitive to 0.1 μg; and there is little adsorption onto the liquid phase. The method has been successfully used for bile, and it has the potential for use on serum.

Keywords

• trifluoroacetates
• bile
• SP-2401 liquid phase
• squirrel monkey