Micro-magnetic resonance imaging of ex vivo mouse embryos with potato starch suspension

Tomokazu Tsurugizawa; Takuma Kumamoto; Yoshichika Yoshioka

STAR Protocols (Sep 2023)

Micro-magnetic resonance imaging of ex vivo mouse embryos with potato starch suspension

Tomokazu Tsurugizawa,
Takuma Kumamoto,
Yoshichika Yoshioka

Affiliations

Tomokazu Tsurugizawa: Human Informatics and Interaction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba 305-8568, Japan; Faculty of Engineering, Information and Systems, University of Tsukuba, Tsukuba 305-8573, Japan; Graduate School of Frontier Biosciences, Osaka University, Suita 565-0871, Japan; Center for Information and Neural Networks (CiNet), Osaka University and National Institute of Information and Communications Technology (NICT), Suita 565-0871, Japan; Corresponding author
Takuma Kumamoto: Developmental Neuroscience Project, Department of Brain & Neurosciences, Tokyo Metropolitan Institute of Medical Science, Tokyo 156-8506, Japan
Yoshichika Yoshioka: Graduate School of Frontier Biosciences, Osaka University, Suita 565-0871, Japan; Center for Information and Neural Networks (CiNet), Osaka University and National Institute of Information and Communications Technology (NICT), Suita 565-0871, Japan; Corresponding author

Journal volume & issue: Vol. 4, no. 3
p. 102483

Abstract

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Summary: Potato starch suspension (PSS) holds promise as a solution to issues, such as air bubbles and specimen motion, associated with micro-magnetic resonance imaging (micro-MRI) of ex vivo embryos. Here, we present a protocol for using PSS when scanning specimens with micro-MRI. We describe steps for preparing samples and potato starch with phosphate-buffered saline. We then detail steps for specimen immersion and micro-MRI scanning. This protocol will enable micro-MRI of not only embryos but also other specimens, such as insects.For complete details on the use and execution of this protocol, please refer to Tsurugizawa et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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