Stem Cell Research (Oct 2024)

An integration-free induced pluripotent stem cell line from a 42-year-old female donor with the APOE-ε2/ε2 allele

  • Lianying Wang,
  • Ruiyun Guo,
  • Xiaohan Zhang,
  • Zijing Zhou,
  • Herman Yao Akogo,
  • Zewei Fu,
  • Yongzhou Song,
  • Ming Li,
  • Jun Ma

Journal volume & issue
Vol. 80
p. 103506

Abstract

Read online

The APOE 4 allele remains the primary genetic risk factor for sporadic Alzheimer’s disease, whereas the APOE 2 allele emerges as a protective factor. Therapeutic approaches in murine models with human APOE alleles, such as modulating APOE levels and converting isoforms, show efficacy. However, there is a lack of in vitro APOE2-mutant human neuronal models. Hence, in this study, we generated human induced pluripotent stem cells (hiPSCs) from the peripheral blood mononuclear blood cells (PMBC) of a 42-year-old female donor carrying the APOE-ε2/ε2 allele. The newly generated hiPSC displayed normal karyotype and could differentiate into three germ layers. Besides, they retained their original genotype and expressed pluripotency markers.