Nature Communications (May 2021)

Structure of the endocytic adaptor complex reveals the basis for efficient membrane anchoring during clathrin-mediated endocytosis

  • Javier Lizarrondo,
  • David P. Klebl,
  • Stephan Niebling,
  • Marc Abella,
  • Martin A. Schroer,
  • Haydyn D. T. Mertens,
  • Katharina Veith,
  • Roland Thuenauer,
  • Dmitri I. Svergun,
  • Michal Skruzny,
  • Frank Sobott,
  • Stephen P. Muench,
  • Maria M. Garcia-Alai

DOI
https://doi.org/10.1038/s41467-021-23151-7
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 15

Abstract

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During clathrin-mediated endocytosis in yeast, endocytic coat adaptors, Sla2 and Ent1, must remain attached to the plasma membrane to transmit force from the actin cytoskeleton required for membrane invagination. Here authors present a cryo-EM structure of a 16-mer complex of the ANTH and ENTH membrane-binding domains from Sla2 and Ent1 bound to PIP2 that constitutes the anchor to the plasma membrane.