Rice extracts (KDML105 and PL2 cultivars) modulate lipid accumulation, oxidative status, and inflammation in oxidative stress-induced 3T3-L1 adipocytes

Abstract

High reactive oxygen species (ROS) level accumulations are coupled with alterations of pro- and anti-inflammatory cytokines, and antioxidant system abnormalities, and they contribute in adipocytes with developing obesity. This study aimed to investigate the effects of rice extracts from KDML105 and PL2 cultivars on lipid accumulation, oxidative status, and inflammatory gene and protein expression in 3T3-L1 adipocytes. Cells were treated with rice extracts (GBR, BR, and WR). GBR and BR extracts significantly decreased intracellular ROS levels and lipid accumulation by DCFH-DA assay and oil-red O staining. Further, qPCR also showed a decrease in the mRNA expression of TNF- and IL-6. Conversely, the rice extracts increased the expressions of antioxidants (Nrf2, HO-1, GPx4, CAT), and the adiponectin gene, and the expression of the adiponectin SOD2 and GPx4 proteins. These results demonstrated an inhibitory effect of both the GBR and BR extracts on lipid accumulation and regulated the expression of oxidative stress, inflammatory genes, and the proteins in H2O2-induced 3T3-L1 adipocytes. Due to anti-adipogenesis and the oxidative status of GABA and -oryzanol, the germinated rice extracts can be served as a beneficial supplement for the prevention and/or treatment of obesity, and oxidative-related diseases.

Keywords

• rice extracts
• lipid accumulation
• oxidative stress
• obesity