Comparison of collection methods for molecular detection of α-herpes viruses and Treponema pallidum, including evaluation of critical transportation conditions

Abstract

The detection of herpes simplex viruses and Treponemal pallidum from genital lesions requires efficient sampling of genetic material for a reliable molecular diagnosis. From 460 patients attending the Public Health clinic, two swabs (dry cotton swabs and Eswabs) per patient were collected in alternating order from the same lesion. Additionally, three storage conditions of Eswabs up to 28 days were evaluated to assess the stability of DNA over time. Out of the 830 PCRs performed, 20 (2.4%) PCRs were discordant between the two swabs. No significant differences were observed between the two sample types. HSV1 and HSV2 could be reliably detected from Eswabs up to 28 days when kept at room temperature. A single swab from a genital lesion is sufficient for reliable diagnosis of α-herpes viruses and Treponemal pallidum, for which both a dry cotton swab or Eswab could be used.

Keywords

• Molecular biology
• Infectious disease
• Public health