Nature Communications (May 2020)
Molecular mechanism of light-driven sodium pumping
- Kirill Kovalev,
- Roman Astashkin,
- Ivan Gushchin,
- Philipp Orekhov,
- Dmytro Volkov,
- Egor Zinovev,
- Egor Marin,
- Maksim Rulev,
- Alexey Alekseev,
- Antoine Royant,
- Philippe Carpentier,
- Svetlana Vaganova,
- Dmitrii Zabelskii,
- Christian Baeken,
- Ilya Sergeev,
- Taras Balandin,
- Gleb Bourenkov,
- Xavier Carpena,
- Roeland Boer,
- Nina Maliar,
- Valentin Borshchevskiy,
- Georg Büldt,
- Ernst Bamberg,
- Valentin Gordeliy
Affiliations
- Kirill Kovalev
- Institut de Biologie Structurale (IBS), Université Grenoble Alpes, CEA, CNRS
- Roman Astashkin
- Institut de Biologie Structurale (IBS), Université Grenoble Alpes, CEA, CNRS
- Ivan Gushchin
- Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology
- Philipp Orekhov
- Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology
- Dmytro Volkov
- Institute of Biological Information Processing (IBI-7: Structural Biochemistry), Forschungszentrum Jülich GmbH
- Egor Zinovev
- Institute of Biological Information Processing (IBI-7: Structural Biochemistry), Forschungszentrum Jülich GmbH
- Egor Marin
- Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology
- Maksim Rulev
- Institute of Biological Information Processing (IBI-7: Structural Biochemistry), Forschungszentrum Jülich GmbH
- Alexey Alekseev
- Institute of Biological Information Processing (IBI-7: Structural Biochemistry), Forschungszentrum Jülich GmbH
- Antoine Royant
- Institut de Biologie Structurale (IBS), Université Grenoble Alpes, CEA, CNRS
- Philippe Carpentier
- European Synchrotron Radiation Facility Grenoble
- Svetlana Vaganova
- Institute of Biological Information Processing (IBI-7: Structural Biochemistry), Forschungszentrum Jülich GmbH
- Dmitrii Zabelskii
- Institute of Biological Information Processing (IBI-7: Structural Biochemistry), Forschungszentrum Jülich GmbH
- Christian Baeken
- Institute of Biological Information Processing (IBI-7: Structural Biochemistry), Forschungszentrum Jülich GmbH
- Ilya Sergeev
- Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology
- Taras Balandin
- Institute of Biological Information Processing (IBI-7: Structural Biochemistry), Forschungszentrum Jülich GmbH
- Gleb Bourenkov
- European Molecular Biology Laboratory, Hamburg unit c/o DESY
- Xavier Carpena
- XALOC beamline, ALBA synchrotron (CELLS)
- Roeland Boer
- XALOC beamline, ALBA synchrotron (CELLS)
- Nina Maliar
- Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology
- Valentin Borshchevskiy
- Institute of Biological Information Processing (IBI-7: Structural Biochemistry), Forschungszentrum Jülich GmbH
- Georg Büldt
- Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology
- Ernst Bamberg
- Research Center for Molecular Mechanisms of Aging and Age-related Diseases, Moscow Institute of Physics and Technology
- Valentin Gordeliy
- Institut de Biologie Structurale (IBS), Université Grenoble Alpes, CEA, CNRS
- DOI
- https://doi.org/10.1038/s41467-020-16032-y
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 11
Abstract
The Na+-pumping KR2 rhodopsin from Krokinobacter eikastus is a light-driven non-proton cation pump whose mechanism of pumping remains to be understood. Here authors solved crystal structures of the O-intermediate state of the pentameric form of KR2 and its D116N and H30A mutants, which sheds light on the mechanism of non-proton cation light-driven pumping.
