Retrovirology (Dec 2007)

Activation of HIV-1 expression and replication by cGMP dependent protein kinase type 1-β (PKG1β)

  • Jeang Kuan-Teh,
  • Yedavalli Venkat RK,
  • Lee Jia

DOI
https://doi.org/10.1186/1742-4690-4-91
Journal volume & issue
Vol. 4, no. 1
p. 91

Abstract

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Abstract The effect of cGMP (cyclic GMP) dependent protein kinase 1-β (PKG1-β) and cGMP analogues on transcriptional activity and replication of human immunodeficiency virus type 1 (HIV-1) was investigated. Transfection of PKG1β expression plasmid increased expression from an HIV-1 LTR-reporter as well as from an infectious HIV-1 molecular clone, pNL4-3. Treatment of HIV-1 AD8-infected monocyte derived macrophages (MDMs) with cGMP agonists and cGMP antagonists caused respectively increased and decreased virus replication. These findings provide evidence that cGMP and PKG serve to regulate HIV-1 infection in human cells.