Engineering cell signaling using tunable CRISPR–Cpf1-based transcription factors

Yuchen Liu; Jinghong Han; Zhicong Chen; Hanwei Wu; Hongsong Dong; Guohui Nie

doi:10.1038/s41467-017-02265-x

Nature Communications (Dec 2017)

Engineering cell signaling using tunable CRISPR–Cpf1-based transcription factors

Yuchen Liu,
Jinghong Han,
Zhicong Chen,
Hanwei Wu,
Hongsong Dong,
Guohui Nie

Affiliations

Yuchen Liu: Institute of Translational Medicine, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University
Jinghong Han: Institute of Translational Medicine, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University
Zhicong Chen: Institute of Translational Medicine, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University
Hanwei Wu: Institute of Translational Medicine, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University
Hongsong Dong: Department of Otolaryngology, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University
Guohui Nie: Institute of Translational Medicine, Shenzhen Second People’s Hospital, The First Affiliated Hospital of Shenzhen University

DOI: https://doi.org/10.1038/s41467-017-02265-x
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 8

Abstract

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Cpf1 has been repurposed as a transcriptional repressor in bacteria and plants. Here, the authors construct activators and repressors in human cells using Cpf1 coupled to riboswitches and GPCRs.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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