Analytical methods for determination of bisphenol A, 4-tert-octylphenol and 4-nonylphenol in herrings and physiological fluids of the grey seal

Abstract

• The aim of this work was to develop the methods of determination for phenol derivatives: bisphenol A (BPA), 4-tert-octylphenol (OP) and 4-nonylphenol (NP), in the whole body of herring Clupea harengus and in physiological fluids of the Baltic grey seal Halichoerus grypus grypus (blood and milk). • Methods were based on liquid chromatography coupled with a fluorescence detector (HPLC-FL). • These methods were satisfactorily validated, each showing good recovery (>80%) and precision (RSD < 15%). Regarding the limit of quantification (LOQ), this was established at <2 ng g−1 for herring, <0.07 ng cm-3 for blood and <0.1 ng cm−3 for milk. Method name: Determination of bisphenol A, 4-tert-octylphenol and 4-nonylphenol in whole fish, blood and milk samples by HPLC-FL, Keywords: Phenol derivatives, Biological samples, Liquid chromatography, Fluorescence detector