A Ca2+-regulated deAMPylation switch in human and bacterial FIC proteins

Simon Veyron; Giulia Oliva; Monica Rolando; Carmen Buchrieser; Gérald Peyroche; Jacqueline Cherfils

doi:10.1038/s41467-019-09023-1

Nature Communications (Mar 2019)

A Ca2+-regulated deAMPylation switch in human and bacterial FIC proteins

Simon Veyron,
Giulia Oliva,
Monica Rolando,
Carmen Buchrieser,
Gérald Peyroche,
Jacqueline Cherfils

Affiliations

Simon Veyron: CNRS and Ecole normale supérieure Paris-Saclay, Laboratoire de Biologie et Pharmacologie Appliquée
Giulia Oliva: Institut Pasteur and CNRS UMR 3525, Biologie des Bactéries Intracellulaires
Monica Rolando: Institut Pasteur and CNRS UMR 3525, Biologie des Bactéries Intracellulaires
Carmen Buchrieser: Institut Pasteur and CNRS UMR 3525, Biologie des Bactéries Intracellulaires
Gérald Peyroche: CNRS and Ecole normale supérieure Paris-Saclay, Laboratoire de Biologie et Pharmacologie Appliquée
Jacqueline Cherfils: CNRS and Ecole normale supérieure Paris-Saclay, Laboratoire de Biologie et Pharmacologie Appliquée

DOI: https://doi.org/10.1038/s41467-019-09023-1
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 10

Abstract

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In many AMPylating FIC proteins a structurally conserved glutamate represses AMPylation. Here, the authors show that this glutamate supports deAMPylation in Enterococcus faecalis FIC (EfFIC), and that EfFIC switches from AMPylation to deAMPylation by binding Ca2+ at distinct sites.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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