Enhanced succinic acid production by Mannheimia employing optimal malate dehydrogenase

Jung Ho Ahn; Hogyun Seo; Woojin Park; Jihye Seok; Jong An Lee; Won Jun Kim; Gi Bae Kim; Kyung-Jin Kim; Sang Yup Lee

doi:10.1038/s41467-020-15839-z

Nature Communications (Apr 2020)

Enhanced succinic acid production by Mannheimia employing optimal malate dehydrogenase

Jung Ho Ahn,
Hogyun Seo,
Woojin Park,
Jihye Seok,
Jong An Lee,
Won Jun Kim,
Gi Bae Kim,
Kyung-Jin Kim,
Sang Yup Lee

Affiliations

Jung Ho Ahn: Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Plus Program), Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST)
Hogyun Seo: School of Life Sciences, KNU Creative BioResearch Group, Kyungpook National University
Woojin Park: School of Life Sciences, KNU Creative BioResearch Group, Kyungpook National University
Jihye Seok: School of Life Sciences, KNU Creative BioResearch Group, Kyungpook National University
Jong An Lee: Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Plus Program), Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST)
Won Jun Kim: Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Plus Program), Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST)
Gi Bae Kim: Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Plus Program), Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST)
Kyung-Jin Kim: School of Life Sciences, KNU Creative BioResearch Group, Kyungpook National University
Sang Yup Lee: Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Plus Program), Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST)

DOI: https://doi.org/10.1038/s41467-020-15839-z
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 12

Abstract

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Malate dehydrogenase (MDH) is one of the key enzymes for succinic acid (SA) bioproduction. Here, the authors report biochemical and structural analyses of various MDHs to reveal amino acids influencing the specific activity and susceptibility to substrate inhibition, and achieve industrial-level SA production.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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