Depletion of <i>Mdig</i> Changes Proteomic Profiling in Triple Negative Breast Cancer Cells

Chitra Thakur; Nicholas J. Carruthers; Qian Zhang; Liping Xu; Yao Fu; Zhuoyue Bi; Yiran Qiu; Wenxuan Zhang; Priya Wadgaonkar; Bandar Almutairy; Chunna Guo; Paul M. Stemmer; Fei Chen

doi:10.3390/biomedicines10082021

Biomedicines (Aug 2022)

Depletion of <i>Mdig</i> Changes Proteomic Profiling in Triple Negative Breast Cancer Cells

Chitra Thakur,
Nicholas J. Carruthers,
Qian Zhang,
Liping Xu,
Yao Fu,
Zhuoyue Bi,
Yiran Qiu,
Wenxuan Zhang,
Priya Wadgaonkar,
Bandar Almutairy,
Chunna Guo,
Paul M. Stemmer,
Fei Chen

Affiliations

Chitra Thakur: Stony Brook Cancer Center, Renaissance School of Medicine, Stony Brook University, The State University of New York, Lauterbur Drive, Stony Brook, NY 11794, USA
Nicholas J. Carruthers: Institute of Environmental Health Sciences, Wayne State University, 2309 Scott Hall, 540 E Canfield Ave, Detroit, MI 48202, USA
Qian Zhang: Department of Pharmaceutical Sciences, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, 259 Mack Avenue, Detroit, MI 48201, USA
Liping Xu: Department of Pharmaceutical Sciences, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, 259 Mack Avenue, Detroit, MI 48201, USA
Yao Fu: Stony Brook Cancer Center, Renaissance School of Medicine, Stony Brook University, The State University of New York, Lauterbur Drive, Stony Brook, NY 11794, USA
Zhuoyue Bi: Stony Brook Cancer Center, Renaissance School of Medicine, Stony Brook University, The State University of New York, Lauterbur Drive, Stony Brook, NY 11794, USA
Yiran Qiu: Stony Brook Cancer Center, Renaissance School of Medicine, Stony Brook University, The State University of New York, Lauterbur Drive, Stony Brook, NY 11794, USA
Wenxuan Zhang: Stony Brook Cancer Center, Renaissance School of Medicine, Stony Brook University, The State University of New York, Lauterbur Drive, Stony Brook, NY 11794, USA
Priya Wadgaonkar: Department of Pharmaceutical Sciences, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, 259 Mack Avenue, Detroit, MI 48201, USA
Bandar Almutairy: Department of Pharmaceutical Sciences, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, 259 Mack Avenue, Detroit, MI 48201, USA
Chunna Guo: Department of Immunology and Microbiology, Wayne State University, Detroit, MI 48201, USA
Paul M. Stemmer: Institute of Environmental Health Sciences, Wayne State University, 2309 Scott Hall, 540 E Canfield Ave, Detroit, MI 48202, USA
Fei Chen: Stony Brook Cancer Center, Renaissance School of Medicine, Stony Brook University, The State University of New York, Lauterbur Drive, Stony Brook, NY 11794, USA

DOI: https://doi.org/10.3390/biomedicines10082021
Journal volume & issue: Vol. 10, no. 8
p. 2021

Abstract

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Triple-negative breast cancers are highly aggressive with an overall poor prognosis and limited therapeutic options. We had previously investigated the role of mdig, an oncogenic gene induced by some environmental risk factors, on the pathogenesis of breast cancer. However, a comprehensive analysis of the proteomic profile affected by mdig in triple-negative breast cancer has not been determined yet. Using label-free bottom-up quantitative proteomics, we compared wildtype control and mdig knockout MDA-MB-231 cells and identified the proteins and pathways that are significantly altered with mdig deletion. A total of 904 differentially expressed (p mdig gene and yielded substantial information on the key proteins, biological processes, and pathways modulated by mdig that contribute to breast cancer tumorigenicity and invasiveness.

Published in Biomedicines

ISSN: 2227-9059 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Biology (General)
Website: http://www.mdpi.com/journal/biomedicines

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