A Study of Biokinetic Peculiarities and Optimization of the Conditions for Culturing Vibrio cholerae Strains Overproducing Protective Antigens Suitable for Use in the Production

Abstract

Described in the work are the major biokinetic criteria of the submerged growth process of V. cholerae Ol strains (classical and eltor biovars), O139, non-Ol and non-O139 serogroups, capable of overproducing the main protective antigens (Ol and O139 antigens, type I and II cholera toxin, cholera toxin type II В subunit, toxin-coregulated adhesion pili). The optimal parameters were defined for each strain (nutritional media, pH indices, cultivation time, application of an additional carbohydrate source) facilitating the maximal yield of the protective antigens under the production conditions, thus making it possible to use them as a basis for manufacturing more efficient vaccinal preparations as well as to obtain the main V. cholerae protective antigens as purified drugs for constructing diagnostic preparations.

Keywords

• <i>vibrio cholerae</i>
• submerged cultivation
• protective antigens