Frontiers in Plant Science (Dec 2015)

Quantitative trait locus analysis of seed germination and seedling vigour in Brassica rapa reveals QTL hotspots and epistatic interactions

  • Ram Kumar Basnet,
  • Ram Kumar Basnet,
  • Anita eDuwal,
  • Dev Nidhi Tiwari,
  • Dong eXiao,
  • Dong eXiao,
  • Sokrat eMonakhos,
  • Sokrat eMonakhos,
  • Johan eBucher,
  • Richard Gerardus Franciscus Visser,
  • Richard Gerardus Franciscus Visser,
  • Steven P. C. Groot,
  • Guusje eBonnema,
  • Guusje eBonnema,
  • Chris eMaliepaard

DOI
https://doi.org/10.3389/fpls.2015.01032
Journal volume & issue
Vol. 6

Abstract

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The genetic basis of seed germination and seedling vigour is largely unknown in Brassica species. We performed a study to evaluate the genetic basis of these important traits in a B. rapa doubled haploid population from a cross of a yellow-seeded oil-type yellow sarson and a black-seeded vegetable-type pak choi. We identified 26 QTL regions across all 10 linkage groups for traits related to seed weight, seed germination and seedling vigour under non-stress and salt stress conditions illustrating the polygenic nature of these traits. QTLs for multiple traits co-localized and we identified eight hotspots for quantitative trait loci (QTL) of seed weight, seed germination, and root and shoot lengths. A QTL hotspot for seed germination on A02 mapped at the B. rapa FLOWERING LOCUS C (BrFLC2). Another hotspot on A05 with salt stress specific QTLs co-located with the B. rapa FATTY ACID DESATURASE 2 (BrFAD2) locus. Epistatic interactions were observed between QTL hotspots for seed germination on A02 and A10 and with a salt tolerance QTL on A05. These results contribute to the understanding of the genetics of seed quality and seeding vigour in B. rapa and can offer tools for Brassica breeding.

Keywords