Metabolomics and amino acid profiling of plasma reveals the metabolite profiles associated with nitrogen utilisation efficiency in primiparous dairy cows

H. Li; S.K. La; L.Y. Zhang; S. Li; Z.B. Yu; L.M. Ao; T.Y. Gao; H.T. Huang

Animal (Oct 2024)

Metabolomics and amino acid profiling of plasma reveals the metabolite profiles associated with nitrogen utilisation efficiency in primiparous dairy cows

H. Li,
S.K. La,
L.Y. Zhang,
S. Li,
Z.B. Yu,
L.M. Ao,
T.Y. Gao,
H.T. Huang

Affiliations

H. Li: College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, Henan Province, PR China; Henan International Joint Laboratory of Nutrition Regulation and Ecological Raising of Domestic Animal, College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, PR China
S.K. La: College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, Henan Province, PR China
L.Y. Zhang: College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, Henan Province, PR China; Henan International Joint Laboratory of Nutrition Regulation and Ecological Raising of Domestic Animal, College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, PR China
S. Li: ZhongLi (Hinggan League) Animal Husbandry Co. LTD, Ulanhot 137400, Inner Mongolia Autonomous Region, PR China
Z.B. Yu: ZhongLi (Hinggan League) Animal Husbandry Co. LTD, Ulanhot 137400, Inner Mongolia Autonomous Region, PR China
L.M. Ao: ZhongLi (Hinggan League) Animal Husbandry Co. LTD, Ulanhot 137400, Inner Mongolia Autonomous Region, PR China
T.Y. Gao: College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, Henan Province, PR China; Henan International Joint Laboratory of Nutrition Regulation and Ecological Raising of Domestic Animal, College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, PR China
H.T. Huang: College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, Henan Province, PR China; Henan International Joint Laboratory of Nutrition Regulation and Ecological Raising of Domestic Animal, College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, 450046, PR China; Corresponding author.

Journal volume & issue: Vol. 18, no. 10
p. 101202

Abstract

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Nitrogen (N) utilisation efficiency (NUE, milk N yield [g/d]/N intake [g/d]) is an important performance indicator in dairy farming. Determining the NUE-associated blood metabolite profile will contribute to the optimisation of nutritional strategies to further improve NUE among dairy cows. Here, 20 primiparous lactating cows with days in milk ranging from 95 to 115 days were selected from a total of 1 221 cows. Each cow’s N intake and milk N yield were measured for 7 days. Subsequently, blood samples were collected before morning feeding. Based on analysis and calculations, cows were retrospectively classified into two groups based on their NUE values, namely, a low NUE group (LNUE, NUE = 24.8 ± 1.6%, n = 10, mean ± SD) and a high NUE group (HNUE, NUE = 35.2 ± 1.7%, n = 10, mean ± SD). Plasma samples were selected from six cows in each group for metabolomics and amino acid profiling. Among the 41 differential metabolites (DMs) identified in the metabolomic analysis, sucrose, MG(0:0/22:1(13Z)/0:0), 2-amino-6-hydroxyhexanoic acid, and L-glutamine exhibited significant correlations with NUE, milk yield, and BW (P 0.1). These results provided new insights into the new blood metabolite profile associated with NUE in dairy cows. These new insights can provide foundational information for the formulation of new strategies to further enhance NUE in dairy cows.

Published in Animal

ISSN: 1751-7311 (Print); 1751-732X (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Agriculture: Animal culture
Website: https://www.journals.elsevier.com/animal/

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