International Journal of Nanomedicine (Apr 2020)

Pumpkin Oil–Based Nanostructured Lipid Carrier System for Antiulcer Effect in NSAID-Induced Gastric Ulcer Model in Rats

  • Ahmed OAA,
  • Fahmy UA,
  • Bakhaidar R,
  • El-Moselhy MA,
  • Alfaleh MA,
  • Ahmed ASF,
  • Hammad ASA,
  • Aldawsari H,
  • Alhakamy NA

Journal volume & issue
Vol. Volume 15
pp. 2529 – 2539

Abstract

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Osama AA Ahmed,1,2 Usama A Fahmy,1 Rana Bakhaidar,1 Mohamed A El-Moselhy,3,4 Mohamed A Alfaleh,5 Al-Shaimaa F Ahmed,4 Asmaa SA Hammad,4 Hibah Aldawsari,1 Nabil A Alhakamy1,6–8 1Advanced Drug Delivery Research Group, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia; 2Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Minia University, Minia 61519, Egypt; 3Department of Pharmacology, School of Pharmacy, Ibn Sina National College, Jeddah 22413, Saudi Arabia; 4Department of Pharmacology and Toxicology, Faculty of Pharmacy, Minia University, Minia 61519, Egypt; 5Department of Natural Products and Alternative Medicine, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia; 6Department of Pharmaceutics, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia; 7Center of Excellence for Drug Research and Pharmaceutical Industries, King Abdulaziz University, Jeddah 21589, Saudi Arabia; 8King Fahd Medical Research Center, King Abdulaziz University, Jeddah 21589, Saudi ArabiaCorrespondence: Osama AA AhmedAdvanced Drug Delivery Research Group, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi ArabiaTel +966 599120686Email [email protected]: Peptic ulcer disease, a painful lesion of the gastric mucosa, is considered one of the most common gastrointestinal disorders. This study aims to investigate the formulation of pumpkin seed oil (PSO)-based nanostructured lipid carriers (NLCs) to utilize PSO as the liquid lipid component of NLCs and to achieve oil dispersion in the nano-range in the stomach.Methods: Box–Behnken design was utilized to deduce the optimum formula with minimum particle size. The optimized PSO-NLCs formula was investigated for gastric ulcer protective effects in Wistar rats by evaluating ulcer index and determination of gastric mucosa oxidative stress parameters.Results: PSO was successfully incorporated as the liquid lipid (LL) component of NLCs. The prepared optimum PSO-NLCs formula showed a size of 64.3 nm. Pretreatment of animals using the optimized PSO-NLCs formula showed significantly (p< 0.001) lower ulcer index compared to indomethacin alone group and significantly (p< 0.05) less mucosal lesions compared to the raw oil.Conclusion: These results indicated great potential for future application of optimized PSO-NLCs formula for antiulcer effect in non-steroidal anti-inflammatory drug (NSAID)-induced gastric ulcer.Keywords: natural products, gastric ulcer, pumpkin seed oil, nano-lipid carriers, optimization, Box–Behnken experimental design

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