Protocol for cryoinjury model in neonatal mice for heart regeneration and repair research

Abstract

Summary: The variability of animal experimental groups and high maternal cannibalization are two major limitations in cardiac injury models. A cryoinjury model could be an ideal model in heart regeneration and repair research as it can provide reproducible results and the injury size can be scaled. Here, we describe a simple and successful cryoinjury model (rate of mouse survival >90% and rate of maternal cannibalization <5%) for evaluating heart injury in regenerating and non-regenerating mice.For complete details on the use and execution of this protocol, please refer to Zhao et al. (2021).

Keywords

• Cell Biology
• Model Organisms
• Tissue Engineering
• Biotechnology and bioengineering